Wheat is one of the primary crops for human nutrition and is used in milling, food, and pharmaceutical industries. In agricultural production, there are various factors that affect the yield and hence financial gain. Therefore, one of the major factors causing significant issues is the preharvest sprouting (PHS). Damages due to the PHS worldwide amount to approximately 1 trillion dollars. The research objectives were as follows: to examine sprouting on spikes after harvest (rated from 1 – 7) and to assess the variability of sprouting traits on spikes using a molecular marker (Vp1B3). Statistically significant differences were detected between the tested wheat cultivars for the sprouting trait on spikes. For most cultivars with a lower sprouting rating on spikes, the presence of the 569 bp allele (PHS tolerance) was detected, while for most cultivars with a high sprouting rating on spikes, the presence of the 652 bp allele (PHS sensitivity) at the Vp1B3 locus was identified.