During the years 2017 and 2018, field studies were carried out on the impact of year, location and interaction of genotype x location and genotype x year on yield, oil and protein content of soybean seed. The experiment included twenty most common soybean genotypes of different maturation groups, which have a 75% share in the sowing structure. The experiment was set up on locations Osijek and Kutjevo, in two repetitions, in a randomized block design. In 2018, an average higher seed yield, oil content and protein content were achieved primarily due to proper distribution of rainfall. Location Osijek in years of research had an average higher seed yield, and oil and protein content. According to the obtained results of the variance analysis for enotype, interaction of genotype x location and genotype x year, statistically highly justified differences (P<0.01) were obtained for seed yield. For genotype and interaction of genotype x year, statistically justified differences (P<0.05) were obtained for the oil and protein content. Research results will contribute to the proper selection of genotypes depending on the purpose of production in order to exploit the genetic potential of the genotype which is most suitable for a particular location.