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The influence of benefit microorganisms on yield and quality of soybean grains under conditions of reduced nitrogen fertilization

Suzana Kristek, Lidija Lenar, Jurica Jović, Tihana Marček, Krunoslav Zmaić, Ivo Rešić, Sanda Rašić  (original scientific paper)


The aim of this study was to investigate the possibility to reduce the application of mineral nitrogen fertilizers through the application of beneficial microorganisms (genus Bradyrhizobium, Azotobacter, bacteria Pseudomonas fluorescens, Bacillus spp., etc.). Research was conducted during 2013 and 2014 on Eutric brown soil. The experiment was set up in a split-block scheme with 12 different variants in 4 repetitions: two soybean cultivars were used; two different treatments of nitrogen fertilizers and three different treatments of microbiological preparation were applied. Analysed parameters were soybean grain yield (kg/ha) based on 13% moisture, protein content (%), oil content (%) and hectolitre mass (kg). Given that the climatic conditions in the second year of research were more favourable than in the first year of research, all the elements of research, including control variants, achieved better results in the second year of research. All variants treated with microbiological preparations, either by application in soil or by application in soil combined with foliar treatments, also achieved statistically significant differences compared to the control variants.

Correspondent author:
Suzana Kristek, Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Vladimira Preloga 1, 31000 Osijek, Croatia,